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Индуцибельные промоторы E. Coli

Anonymous, 16.12.2001 01:33
Занимается ли кто-нибудь регуляцией экспрессии генов E. Coli? Мне нужно выбрать какой-нибудь небанальный промотор, для которого легко смоделировать условия индукции и репрессии. Может что-нибудь посоветуете? Заранее спасибо.
Anonymous, 16.12.2001 20:37
Именно небанальный? А чем тебе lac промотор плох? Или уж для точной репресии (lac и его производные могут течь) можно и промотор Т7 фага, его тоже для этого часто пользуют.
Anonymous, 17.12.2001 23:05
Мы хотим проверить, принимает ли наш белок участие в репрессии РАЗНЫХ промоторов, поэтому хотелось бы знать, работал ли кто-нибудь с чем-либо кроме Lac (м.б. с температурной индукцией и т.п.)
Казалось бы, уж E. coli - вдоль и поперек изучили, а информации мало...
Anonymous, 18.12.2001 12:39
Продаю идею: клонируйте свой белок на разных плазмидах и делайте array на все гены колей.
А если насчет промотора - то PL,PR - фага лямбды, арабинозный промотор...
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