Коллеги! Может ли быть такое, что в клетках E.Coli, не DE3, без хромосомной копии T7РНК полимеразы, без индукции фагом и без добавления ИПТГ наблюдается экспрессия с T7 промотора? У меня наблюдается. Кто поможет разобраться?
Anonymous, 04.09.2001 10:55
А может у Вас течет не Т7, а промотор ампициллина? В векторах серии pET такое наблюдается...
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