Добрый день.
Внезапно возникла проблема разделения аллелей с разницей до двух нуклеотидов, диапозон 200-250 нуклеотидов. Вроде бы очевидно что ПААГ а не агароза, но для меня это абсолютно новая задача и я прошу совета в отправной точке в поиске методики - посоветуйте, пожалуйста, с чего начать. Понимаю что все начинается с Остермана но подскажите в какой из его книг изложен именно этот вопрос? Может порекомендуете еще что-то или поделитесь своим опытом. Заранее благодарен.

Если до двух нуклеотидов, то это если и ПААГ, то секвенирующий. Т.е. очень тонкий, с высоким вольтажём, с длинным пробегом и с радиоавтографией. Сейчас об этом помнят только старики, учившиеся по Остерману.
Разницу в 1...2 нуклеотида определяют при помощи капиллярных секвенаторов или NGS. Можно и при помощи ПЦР (лучше - Real-Time PCR). Но для этого придётся разработать (или купить) тест-систему.

Да лааадно нагнетать то Вполне себе и весьма молодые гоняют. Рабочий метод

Ну и то ли на ютубчике, то ли на jove видео обучающие попадались от и до как практически "на коленке" это дело ставить, а затем - проявлять

В целом - тоненький хрупкий 6% гель, буферная система дял ДНК, длинный сендвич.
На еБее попадаются хоеферовские или аналогичные камеры под енто дело.

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