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РнК

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Лилу, 20.11.2020 16:36
Кто как выделяет РНК?
MMM, 21.11.2020 00:10
Я выделяю на протяжении долгого времени тризолом и регулярно все выходит. Как жить? smile.gif
Что у вас за бактерии? Это чистая культура? На самом деле тризол хуже выделяет, чем первоначальный реагент Хомченского, кажется он назывался РНК-реагент. Но это не суть, как важно, потому что тризол тоже хорошо выделяет РНК. Тут вопросы дополнительные к первому про культуру бактерий. Какую РНК вы выделяет и для чего, что вы потом с ней делаете.
MMM, 21.11.2020 18:10
R.ruber - это клещевина? И как давно она стала бактерией?
Что значит "для экспрессии"? Вы хотите ген клонировать в векторе для экспрессии?

У меня вопрос: в выделяемом препарате на агарозном форезе видна рибосомная РНК?
genseq, 22.11.2020 09:08
https://microbewiki.kenyon.edu/index.php/Rhodococcus_ruber
Rhodococcus ruber is a gram positive bacteria that is non-motile and non-spore forming. Key features of its cell structure include its thick peptidoglycan layer, mycolic acids, lipoglycans, and cell envelope lipids ...
... it can be a key player in bioremediation efforts to accelerate the degradation of environmental contaminants.
KXL, 26.01.2021 01:37
(MMM @ 21.11.2020 19:10)
Ссылка на исходное сообщение  R.ruber - это клещевина? И как давно она стала бактерией?
Что значит "для экспрессии"? Вы хотите ген клонировать в векторе для экспрессии?

У меня вопрос: в выделяемом препарате на агарозном форезе видна рибосомная РНК?

мммыч если уж рибосомалки нет lol.gif
guest: mahi01 , 18.02.2021 12:16
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guest: mahi01 , 18.02.2021 12:17
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guest: mahi01 , 18.02.2021 12:18
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guest: mahi01 , 18.02.2021 12:18
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guest: mahi01 , 18.02.2021 12:18
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guest: mahi01 , 18.02.2021 12:18
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guest: mahi01 , 18.02.2021 12:18
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guest: mahi01 , 18.02.2021 12:18
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guest: mahi01 , 18.02.2021 12:19
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guest: mahi01 , 18.02.2021 12:19
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guest: mahi01 , 18.02.2021 12:19
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guest: mahi01 , 18.02.2021 12:19
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guest: mahi01 , 18.02.2021 12:19
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guest: mahi01 , 18.02.2021 12:20
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guest: mahi01 , 18.02.2021 12:20
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guest: mahi01 , 18.02.2021 12:20
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guest: mahi01 , 18.02.2021 12:20
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guest: mahi01 , 18.02.2021 12:20
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guest: mahi01 , 18.02.2021 12:20
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guest: mahi01 , 18.02.2021 12:20
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guest: mahi01 , 18.02.2021 12:20
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guest: mahi01 , 18.02.2021 12:21
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guest: mahi01 , 18.02.2021 12:21
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guest: mahi01 , 18.02.2021 12:21
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guest: mahi01 , 18.02.2021 12:21
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guest: mahi01 , 18.02.2021 12:21
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guest: mahi01 , 18.02.2021 12:21
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guest: mahi01 , 18.02.2021 12:22
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guest: mahi01 , 18.02.2021 12:22
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guest: mahi01 , 18.02.2021 12:22
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guest: mahi01 , 18.02.2021 12:22
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