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Anonymous, 04.08.2001 19:05
Господа ученые! Практикуюсь с клонированием генов E.coli в BamHI caйт pBR322 методом "shotgun". Хромосома по Sau3A или BamHI bkb BglII, лигирую с вектором и трансформирую клетки с дефектным геном.Отбор трансформантов по компенсации ауксотрофности(из-за дефектного гена).
Может кто-нибудь поделится опытом или советом, т.к. нужнен высокий выход трансформантов.
Заранее благодарен.
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