Чем можно заменить 6M NaI при выделении ДНК из агарозы при помощи стекла?

Original'nyi Geneclean ispol'zuet sorbtsiu DNA na silica v prisutstvii iodida natriya. Poslednii sam po sebe ne stabilen. Drugie kompanii vypuskaut kity s chloratom natriya (u QIAGENa byl kit gde vtori rastvor byl s khloratom natriya, a pervyi - smes' iodida i khlorata) ili s khloridom guanidina.

NaI используется в данной методе, как сильно ионизированный хаотроп, который с одной стороны разрушает водородные связи агарозы, а с другой способствует иммобилизации ДНК на стекле. Поэтому,
в принцепе, подходят любые хаотропные
соли. Например: роданид калия(KCNS),
трихлорацетат натрия - это легко-доступные в России и дешевые; гуанидиния хлорид, гуанидиния роданид(он же тиоцианат, ГТЦ) - это дорогие и только забугорные. Рабочие концентрации сказать не могу никогда не подбирал, но если бы подбирал, то начал с 4М и повышал бы. Если не влом, можете повозится с разными реагентами, а потом
прислать на этот сайт самый лучший вариант.

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