Если кто работал с плазмидой pGEX-5X13, подскажите, пожалуйста, карту или где найти, я знаю только то, что там есть GST.

А она точно называется 5X-13?
Обычно бывает по три серии с разными рамками. Могу предложить карту pGEX-5X-1,2,3

Dorogaya Pola,
To, chto Vy znaete, eto uje ochen' mnogo!
Ved' u Vas est' komputer s dostupom v Internet!
Zakhodite na Altavista ili Yahoo i ischite k primeru "pGEX + plasmid". Vot i vsyo!
Takim obrazom mojno uznat', chto plazmidy eti prodavala Pharmacia, a teper' prodayot AmershamPharmacia Biotech, chto vsego ikh 13 shtuk, no "pGEX-5X13" - ne byvaet.
Tam je (www.apbiotech.com) karty, metodiki, sovety.... Vsyo chto ugodno.

Спасибо большое за советы. Я нашла карту на сайте Pharmacia Amercham.В номере плазмиды действительно ошибка - не 13, конечно, а 3.
Уважаемый Grunt, мне показалось, что Вы работаете с GST-fusion белками. Буду Вам очень благодарна, если Вы поделитесь методиками по экспрессии эукаритического белка в такой конструкции в E.coli, его выделению в больших количествах и очистке.

Пола, Я хотел Вам отправить письмо. Но Ваш ящик на imail полон.
П.С.
А это правда, что Вы занимаетесь ДНК полимеразой альфа?

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