Ищу различные методики для определения протеинкиназной активности.

Я делаю реакцию фосфорилирования target-пептида меченным фосфором. Потом считаю количество встроенного фосфора и таким образом определяю активность киназы. Если интересно, отвечу поподробнее.

Go to http://stke.sciencemag.org/,
then click on "protocols"
http://stke.sciencemag.org/cgi/keSearch?RE...ction=protocols
You're gonna find piles of kinase assays, prots etc. Enjoy yourself.

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